AmpliMERGE - Amplicon Sequencing MERGing tool
AmpliMERGE merges paired-end reads from next-generation sequencing experiments.
Some NGS technologies can read amplicon sequences from both sides (covering partially or totally the
amplified region) and retrieve paired-end reads that overlap (e.g. Illumina Mi-Seq). These paired-end reads must
be merged into a unique sequence before continuing the data analysis. The merging process will correct errors in
low quality sequenced positions and will increase the length of the sequence if it was not fully covered by any
of the paired-end reads.
AmpliMERGE runs in background the FLASH algorithm with optimum overlapping parameters:
AmpliMERGE takes as input:
- SEQUENCE FILE R1: First paired-end reads file in FASTQ or FASTA format (compressed or uncompressed). Multiple files should be named '*_R1.*' and packed into a unique .ZIP or .TAR.GZ file.
- SEQUENCE FILE R2: First paired-end reads file in FASTQ or FASTA format (compressed or uncompressed). Multiple files should be named '*_R2.*' and packed into a unique .ZIP or .TAR.GZ file.
- AMPLICON DATA *: primer and tag information in a CSV format file as explained in the documentation.
Results can be downloaded on the same page or from an email message after analysis completion.
Output files will be in FASTQ compressed format, ready to be used in other AmpliSAT tools like AmpliCHECK or AmpliSAS.
For more information, read the documentation.
Run AmpliMERGE
Disclaimer
Your use of any of these tools is at your own risk. We do not give any representation or warranty nor assume any liability or responsibility for the data nor the results posted (whether as to their accuracy, completeness, quality or otherwise). Access to these data is available free of charge for ordinary use in the course of research. By visiting the site, you accept our use of cookies and you accept that your data and results will be stored in our server.